Viability of Gyr (Bos indicus) in vitro produced embryos after vitrification under field conditions

  • Lucas Carvalho Pereira Nordeste in vitro, Maceió, AL
  • José Carlos Ferreira-Silva Universidade Federal Rural de Pernambuco, Recife, PE
  • Ludymila Furtado Cantanhêde Universidade Federal Rural de Pernambuco, Recife, PE
  • Humberto Fernandes Veloso Neto Universidade Federal Rural de Pernambuco, Recife, PE
  • Joaquim Corrêa Andrade Universidade Federal Rural de Pernambuco, Recife, PE
  • Bartira Pastor de Andrade Souza Universidade Federal Rural de Pernambuco, Recife, PE
  • Marcelo Tigre Moura Universidade Federal Rural de Pernambuco, Recife, PE
  • Marcos Antônio Lemos de Oliveira Universidade Federal Rural de Pernambuco, Recife, PE
Palavras-chave: Bos indicus, cryobiology, cryotolerance, Zebu.

Resumo

The aim of this study was to evaluate the pregnancy rate of in vitro produced embryos of Gyr cattle (Bos indicus) after cryopreservation by the vitrification method under field conditions. Blastocysts in different developmental stages were transferred to recipient cows either fresh (n = 140) or warmed after vitrification (n = 138). The pregnancy rates obtained for fresh embryos were 46.15% (initial blastocyst), 46.93% (blastocyst) and 50.0% (expanded blastocyst) at 35 days post-fertilization, and 43.58% (initial blastocyst), 46.93% (blastocyst) and 50.0% (expanded blastocyst) at 60 days. The pregnancy rates after embryo vitrification were 35.0% (initial blastocyst), 42.3% (blastocyst) and 43.47% (expanded blastocyst) at 35 days post-fertilization, and 32.5% (initial blastocyst), 38.46% (blastocyst) and 43.47% (expanded blastocyst) at 60 days. Embryo vitrification or blastocyst development stage did not affect pregnancy rates or the incidence of embryonic death. In conclusion, vitrification of Gyr (Bos indicus) embryos under field conditions is an efficient method that can be implemented to use surplus in vitro produced embryos without affecting pregnancy rates. 

Publicado
29-06-2016
Como Citar
Pereira, L., Ferreira-Silva, J., Cantanhêde, L., Veloso Neto, H., Andrade, J., Souza, B., Moura, M., & Oliveira, M. (2016). Viability of Gyr (Bos indicus) in vitro produced embryos after vitrification under field conditions. Boletim De Indústria Animal, 73(2), 159-164. https://doi.org/10.17523/bia.v73n2p159
Seção
REPRODUÇÃO ANIMAL